Food Processing: Techniques and Technology Food Processing: Techniques and Technology Техника и технология пищевых производств 2074-9414 2313-1748 96600 10.21603/2074-9414-2025-1-2563 ОРИГИНАЛЬНАЯ СТАТЬЯ ORIGINAL ARTICLE ОРИГИНАЛЬНАЯ СТАТЬЯ Anti-Metabolic Syndrome Effect of Trans-Cinnamic Acid Потенциал транс-коричной кислоты для профилактики метаболического синдрома https://orcid.org/0009-0002-3826-8048 Чекушкина Дарья Юрьевна Chekushkina Darya Yu. chekushkina02@mail.ru https://orcid.org/0000-0002-8071-4411 Федорова Анастасия Михайловна Fedorova Anastasia M. https://orcid.org/0009-0006-2889-4458 Коваленко Светлана Викторова Kovalenko Svetlana V. https://orcid.org/0000-0002-3536-562X Милентьева Ирина Сергеевна Milentyeva Irina S. https://orcid.org/0000-0001-7035-673X Альтшулер Ольга Генриховна Altshuler Olga G. Аксенова Лариса Михайловна Aksenova Larisa M. Кемеровский государственный университет Кемерово Россия Kemerovo State University Kemerovo Russian Federation Кемеровский государственный университет Кемерово Россия Kemerovo State University Kemerovo Russian Federation Кемеровский государственный университет Кемерово Россия Kemerovo State University Kemerovo Russian Federation Кемеровский государственный университет Кемерово Россия Kemerovo State University Kemerovo Russian Federation Кемеровский государственный университет Кемерово Россия Kemerovo State University Kemerovo Russian Federation Всероссийский научно-исследовательский институт кондитерской промышленности Москва Россия All-Russian Scientific Research Institute of Confectionery Industry Moscow Russian Federation 28 03 2025 28 03 2025 55 1 136 147 23 12 2024 29 01 2025 https://fptt.ru/en/issues/23416/23396/

Метаболический синдром, также известный как синдром Х или синдром резистентности к инсулину, является глобальной проблемой человечества, характеризующейся висцеральным ожирением, резистентностью к инсулину, высоким кровяным давлением, гипогликемией, гипохолестеринемией и сердечно-сосудистыми заболеваниями. Цель данного исследования – изучить потенциал профилактики метаболического синдрома с помощью смесей биологически активных веществ, состоящих из рутина (RUT), кверцетина (KVC) и транс-коричной кислоты (TKR-k), в составе которых преобладает TKR-k. Объектами исследований послужили смеси биологически активных веществ, включающие транс-коричную кислоту, рутин и кверцетин в различных соотношениях: смесь № 1 – RUT:KVC:TKR-k в соотношении 1:1:2, смесь № 2 – RUT:TKR-k в соотношении 1:3, смесь № 3 – RUT:KVC:TKR-k в соотношении 4:1:15, смесь № 4 – KVC:TKR-k в соотношении 3:1. Определение гипохолестеринемической активности исследуемых растворов биологически активных веществ в эффективной дозе 100,0 мг/кг осуществлялось на модельных объектах (самцах черных мышей), у которых моделировали гиперхолестеринемию введением ингибитора липопротеинлипазы – полоксамера P407 в эффективной дозе 400,0 мг/кг. Определение гипогликемической активности проводилось in vivo. За час до запланированного времени введения смесей модельным объектам проводилась предварительная подготовка, в процессе которой каждую крысу взвешивали и подбирали индивидуально необходимую концентрацию смесей в эффективной дозе 100,0 мг/кг, глибенкламида – 5,0 мг/кг и глюкозы – 2000,0 мг/кг. Затем производили забор крови из кончика хвоста для протоколирования входных данных об уровне глюкозы и общего холестерина. По результатам исследования выявлено достоверное снижение площади под кривой «концентрация глюкозы – время» (смесь № 3) (p < 0,01), что свидетельствует о гипогликемической активности. Зафиксирована тенденция снижения глюкозы во всех экспериментальных группах, однако достоверно уровень глюкозы в данной модели был снижен только в смеси № 3. Установлено, что смеси № 1–4 обладают выраженным достоверным гипохолестеринемическим эффектом. При определении уровня триглицеридов и липопротеинов низкой плотности статистически значимых различий между группами обнаружено не было, однако наблюдались тренды снижения триглицеридов в смесях № 2 и 3 и липопротеинов низкой плотности (смеси № 1 и 4). Полученные результаты свидетельствуют о том, что целесообразнее использовать смеси № 2–4 как источники для создания биологически активных добавок, направленных на профилактику метаболического синдрома.

The metabolic syndrome, also known as syndrome X or insulin resistance syndrome, is a global human health issue. It is associated with visceral obesity, insulin resistance, high blood pressure, hypoglycemia, hypocholesterolemia, and cardiovascular diseases. This article describes the anti-metabolic syndrome effect of several biologically active mixes that consisted of rutin, quercetin, and trans-cinnamic acid. The experimental mixes differed in composition and ratio, with trans-cinnamic acid being the most abundant component. Mix 1 included rutin, quercetin, and trans-cinnamic acid (1:1:2), Mix 2 consisted of rutin and trans-cinnamic acid (1:3), Mix 3 was a combination of rutin, quercetin, and trans-cinnamic acid (4:1:15), Mix 4 consisted of quercetin and trans-cinnamic acid (3:1). The effective dose was 100.0 mg/kg for all samples. The hypocholesterolemic activity was studied on 48 male black C57Bl/6 mice with hypercholesterolemia induced by lipoprotein lipase inhibitor Poloxamer 407 (400.0 mg/kg). The hypoglycemic activity was determined in vivo on 42 white Wistar rats. Each rat was administered with an individual concentration of the experimental mix (effective doses: 100.0 mg/kg for the mixes, 5.0 mg/kg for glibenclamide, and 2 000.0 mg/kg for glucose). Blood was sampled from the tip of the tail to record the input data on glucose and total cholesterol. The study revealed a reliable decrease (p < 0.01) in the area under curve for glucose concentration and time (Mix 3), which indicated hypoglycemic potential. All groups demonstrated a certain decrease in glucose, but it was statistically significant only in the animals that received Mix 3. All the mixes exhibited a reliable hypocholesterolemic effect. The tests on triglycerides and low-density lipoproteins revealed no statistically significant differences between the experimental groups. However, those treated with Mixes 2 and 3 demonstrated a trend towards lower triglycerides, and those that received Mixes 1 and 4 had a lower level of low-density lipoproteins. Mixes 2 (rutin + trans-cinnamic acid, 1:3), 3 (rutin + quercetin + trans-cinnamic acid, 4:1:15), and 4 (quercetin + trans-cinnamic acid, 3:1) proved to be suitable for anti-metabolic syndrome bioactive additives.

 Транс-коричная кислота кверцетин рутин метаболический синдром профилактика in vivo мыши Trans-cinnamic acid quercetin rutin metabolic syndrome prevention in vivo mice Работа выполнена в рамках государственного задания по теме «Разработка биологически активных добавок, состоящих из метаболитов растительных объектов in vitro, для защиты населения от преждевременного старения» (проект FZSR-2024-0008) с использованием оборудования ЦКП «Инструментальные методы анализа в области прикладной биотехнологии» на базе КемГУ . The research was part of State Assignment FZSR-2024-0008: Anti-aging biologically active additives with plant metabolites in vitro. The experiments were performed at the R&D Center for Instrumental Analysis in Applied Biotechnology, Kemerovo State University , Kemerovo, Russia.

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