Кемерово, Россия
Кемерово, Россия
Новосибирск, Россия
Кемерово, Россия
Кемерово, Россия
Злаковые культуры играют ключевую роль в обеспечении продовольственной безопасности, однако интенсивное применение агрохимикатов сопровождается деградацией почвенных экосистем и ростом экологических рисков. В связи с этим возрастает интерес к микробным агентам биологического контроля. Представители рода Pantoea рассматриваются как перспективные бактерии, стимулирующие рост растений и подавляющие фитопатогены, несмотря на это, их практическое применение требует молекулярно-генетической оценки биобезопасности и метаболического потенциала. Цель исследования – провести полногеномный анализ и биоинформатическую характеристику кластеров биосинтеза вторичных метаболитов штамма Pantoea pleuroti В-14756 для оценки его генетического и метаболического потенциала в качестве агента биоконтроля фитопатогенов злаковых культур. Объектом исследования являлся штамм P. pleuroti В-14756, депонированный в Национальном биоресурсном центре Всероссийской коллекции промышленных микроорганизмов НИЦ «Курчатовский институт» (Москва, Россия). Полногеномное секвенирование выполняли на платформах MGIseq-2000 и Polyseq One. Сборку генома de novo, полировку и аннотацию осуществляли с использованием биоинформатических инструментов Flye, Raven, Trycycler, Medaka, Polypolish, POLCA, BUSCO, Bakta и antiSMASH. В результате секвенирования и сборки получен геном P. pleuroti В-14756 размером 4 735 406 п.н. с GC-составом 53 %. Аннотация генома выявила 4416 кодирующих последовательностей, 7 кластеров рРНК и 78 тРНК. С использованием antiSMASH идентифицировано 9 кластеров биосинтеза вторичных метаболитов, включая кластеры, ассоциированные с синтезом аэробактина, десферриоксамина Е и каротиноидов. Аэробактин и десферриоксамин Е обладают способностью к связыванию, хелатированию и последующему импорту ионов железа, тогда как каротиноиды выполняют антиоксидантную функцию, защищая клетку от активных форм кислорода в стрессовых условиях. Обнаружение у P. pleuroti В-14756 биосинтетических кластеров вторичных метаболитов (NRPS, RiPP, фосфонатных и терпеновых путей) подтверждает выраженный антагонистический потенциал штамма и перспективность применения в биологическом контроле фитопатогенов.
Pantoea pleuroti, биологический контроль, вторичные метаболиты, полногеномное секвенирование, биоинформатический анализ, сидерофоры, антагонистическая активность
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